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Fig. 2 | BMC Biotechnology

Fig. 2

From: Purification of the recombinant green fluorescent protein from tobacco plants using alcohol/salt aqueous two-phase system and hydrophobic interaction chromatography

Fig. 2

Analysis of accumulation of recombinant GFP in N. benthamiana. a Representative TMV vector-infiltrated N. benthamiana leaves under UV-illumination at 8 days post-inoculation (dpi). b Confocal microscopy image of GFP in infiltrated leaves. Green signal in image is due to GFP fluorescence. c Coomassie-stained polyacrylamide gel showing GFP accumulation in infiltrated N. benthamiana leaves. M, protein molecular weight marker in kDa; H, total soluble protein extracts from non-inoculated leaves, negative control; lane 4, 6 and 8, extracts from infiltrated leaves at 4, 6 and 8 dpi, respectively. d Western blot analysis of GFP from infiltrated N. benthamiana leaves. M, protein molecular weight marker in kDa; H, total soluble protein extracts from non-inoculated leaves, negative control; lane 4, 6 and 8, extracts from infiltrated leaves at 4, 6 and 8 dpi, respectively

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BMC Biotechnology

ISSN: 1472-6750

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