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Fig. 1 | BMC Biotechnology

Fig. 1

From: Purification of the recombinant green fluorescent protein from tobacco plants using alcohol/salt aqueous two-phase system and hydrophobic interaction chromatography

Fig. 1

Structures of the expression vectors used in this study. pJL TRBO-G, TMV-based vectors used to express green fluorescent protein (GFP) in N. benthamiana leaves; RB, the right T-DNA border; P35S, duplicated Cauliflower mosaic virus (CaMV) 35S promoter; Replicase, RNA-dependent RNA polymerase of TMV; sg1 and sg2, subgenomic mRNA1 and mRNA2 promoter of the TMV; MP, movement protein of TMV; GFP, green fluorescent protein; Rz, ribozyme; T35S, CaMV polyA signal sequence/terminator; LB, the left T-DNA border; pCBNoX P19, RNA silencing suppressor expression vector used to co-infiltrate with pJL TRBO-G; TE, translational enhancer of Tobacco etch virus (TEV); P19, 19-kDa RNA silencing suppressor from TBSV; Pac I, Not I, Nco I and Xba I, restriction enzyme recognition sites

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BMC Biotechnology

ISSN: 1472-6750

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