Fig. 2

D. suzukii β2t gene and its expression. a Ds_β2t gene has two exons and one intron similar to D. melanogaster. The gene is slightly longer in D. suzukii due to increase in the size of the 5’UTR and the intron. The numbers indicate the first nucleotide of the respective feature relative to the first transcribed nucleotide. b Testes whole mount in situ hybridization using DIG labeled RNA antisense probe against Ds_β2t 5’UTR and exon I detects strong and testes-specific expression. The gene is not expressed at the tip of the testes (black triangle) where stem cells reside. c Negative control using DIG labeled sense probe shows no signs of staining. The abbreviations Tt and Ag refer to testes or the accessory glands, respectively