Table 1 Setup of the horizontal transfer experiment
Substrates and other contamination sources of Ca. E. dacicola | Contamination phase | Acquisition phase |
|---|---|---|
Olives | Exposed to wild flies for 7 days | 25 lab males + 25 lab females per cage exposed for 15 days to 30 putatively contaminated olives |
Gelled water | Exposed to wild flies for 3 days | 25 lab males + 25 lab females per cage exposed for 15 days to putatively contaminated gelled water |
Wax domes | Exposed to wild flies for 24 h | 25 lab males + 25 lab females per cage exposed for 15 days to a wax dome bearing eggs laid by wild females |
Faeces | Approximately 100 wild symbiotic flies per cage for 30 days | 25 lab males + 25 lab females per cage exposed for 15 days to faeces dropped by wild flies |
labF x wildM | Wild naturally symbiotic males | 25 wild males + 25 lab females in cohabitation for 15 days |
labM x wildF | Wild naturally symbiotic females | 25 lab males + 25 wild females in cohabitation for 15 days |