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Table 1 Setup of the horizontal transfer experiment

From: Horizontal transfer and finalization of a reliable detection method for the olive fruit fly endosymbiont, Candidatus Erwinia dacicola

Substrates and other contamination sources of Ca. E. dacicolaContamination phaseAcquisition phase
OlivesExposed to wild flies for 7 days25 lab males + 25 lab females per cage exposed for 15 days to 30 putatively contaminated olives
Gelled waterExposed to wild flies for 3 days25 lab males + 25 lab females per cage exposed for 15 days to putatively contaminated gelled water
Wax domesExposed to wild flies for 24 h25 lab males + 25 lab females per cage exposed for 15 days to a wax dome bearing eggs laid by wild females
FaecesApproximately 100 wild symbiotic flies per cage for 30 days25 lab males + 25 lab females per cage exposed for 15 days to faeces dropped by wild flies
labF x wildMWild naturally symbiotic males25 wild males + 25 lab females in cohabitation for 15 days
labM x wildFWild naturally symbiotic females25 lab males + 25 wild females in cohabitation for 15 days