Fig. 6

PCR-DGGE profiles of the 16S rRNA gene fragments obtained by amplification of DNA extracted from the oesophageal bulb of wild B. oleae flies and B. oleae flies developed from eggs externally treated with SHTX (1% sodium hypochlorite + 0.1% Triton X mixture). DGGE denaturing gradient 48–65%. Arrowed bands indicate band excised; GenBank accession number and % sequence similarity of the nearest BLAST match are also reported. L, ladder; M, 16S rRNA gene fragment obtained by amplification of DNA extracted from the oesophageal bulb of a wild fly and used as marker of Ca. Erwinia dacicola; EM2, EM25–27, sample codes