Fig. 1

PCR-DGGE profiles of the 16S rRNA gene fragments obtained by amplification of DNA extracted from egg samples and rinse water. DGGE denaturing gradient 42–68%. Arrowed band indicates a DNA fragment obtained by amplification of DNA extracted from wild fly oesophageal bulbs and used as species marker of Ca. E. dacicola. L, ladder; M, 16S rRNA gene fragment obtained by amplification of DNA extracted from the oesophageal bulb of a wild fly and used as marker of Ca. Erwinia dacicola; CE, eggs washed with water (control eggs); PAE, eggs treated with 0.3% propionic acid; SHTXE, eggs treated with sodium hypochlorite + Triton X mixture; CW, water from control eggs; PAW, second rinse water after treatment with PA; SHTXW, second rinse water after treatment with SHTX