Skip to main content

Table 1 Top 40 most frequent sequences from the naive library and after 2 rounds of biopanning

From: The next generation of biopanning: next gen sequencing improves analysis of bacterial display libraries

 Naive LibraryNo. SeqsRound 1No. SeqsRound 2No. Seqs
1L32VLPQTSFFAATCRRS115CHISPEKRRLIVCAD4619
2P31SNRLRGENLTFRRHK110YPLCSTYGNVLVGGS3989
3R30DRRRKIERYDAQISV89PLKQGIYETTSTKGT3321
4S22T87YGSRTFPVCSTVHRR2185
5A19S84YRLMEMEEYHKDVFA1985
6T13PAKGSIKNPILLGIR74KHLLCVMQGGTAFRG1783
7E12QPLSESLEDKTSIDV74RIFGFLSCKPNIIKL1094
8H11SS73IVNRMWCLISVISQA921
9SP11THNARRLMPCIDASD73FWGLYPLFCCTGCSP909
10Y11YPGRMEALFAHVSNA73SKLFSSWVLCACWHC778
11Q10RDPSWRAETVNCGNL69AVLAIYPNSLYWLQA656
12TT10RHQNPQEEI69YIAMILHGFSQAFPG650
13V10YTYHGRNSERLLRMK69LLFSFFNCFTGNDGQ636
14F9TQDETRRGGMSTTSS68NKWSPRTDFNTESVR623
15GPCLETCLTA9HTYCVHPSMATCWHY66HAPWECLISVLASAH616
16ERSVG8ANLPDSHHIAHNKDT65ARGLLTAISFYAFQC602
17LT8AMPDFVILDHGVTRR64RLWMRSPTSTAGGIR601
18PRKTLKGTLTVPSYI8KNSLTCCYG63AERLHQITVMLLRSF595
19SG8RRNYIHIGAKAAGRS63LSNLLHYLYALGQPG595
20SKAPLRKTNMKLR8DVLAKYSSLERQDLP62PILLCYVLYCEYASS587
21TS8SVAATCWRYWYDKPL62SWTMLICQLHSYLYT583
22ATYYLQPYVPISEET7RPPCEEMSINCPGVT61PRALWEASMVVTLAC581
23DQLGVL7TYPCIYA61AVCKPVGPKAPWRDR575
24GPWATWSSYDKWHNR7VSLIDQCDCTMQSNP61SSRSLQQNSISRLSM571
25GT7HRRRKPIHLPEYPLP60IRKQWDFLCLGFLLS559
26KS7RHSICPRFRGLLPHS60SAFPACGLASYFPWF554
27PCNFISKHFVPHRRH7RML60GVVRHCPAWQKSPWP532
28PIVGVYK7ARTSG59HSALDVLSWVISSLM532
29PR7DWMKFHVEA59GLRIQAYALCYWEPV526
30SFLL7GPANWTCAWQAVTAF59NRAIIGEIAGLIKFL522
31TD7LLVKLGP59PAAAFFTQVMSLLRQ503
32WD7RGVGTSHPQSVCVKP59RLHCLERYINLAFLP503
33YT7CIT58RMHHLMFRDSHSHTD502
34AMTSLPSTCRQLHSN6WLHGVLLNFKKHGVC58TQKHKTLCFSIRKPG494
35AQDRVRLKSFGRASL6IHSAGRMSINLCVGI57LRYGIWQVSHIALTS492
36CQFNVWANLARIAIG6IRRTLLGRPVWFFEE57QSFVMESVLSFGFSF492
37EDGPTRLWPLSRAAW6RSSRSVDPLTYRRNA57DRRRKIERYDAQISV486
38FSLTVFVMDQENVNR6YQPWREDRRSSAGSA57PHKLWRLGCFVAMDL485
39GDSEGLRMSTLGSSS6GLVRVFWPTPERGHR56KTVFCLTVLPRFEAA482
40GTIGFLDLPRKTVMR6AVKAYSTFRQSPTRL55RHLVSWLCTVLHPDP481