Fig. 1

pB33-dsRed-eCPX3.0 Peptide Library Design. a Plasmid map of DsRed-peptide display expression plasmid showing relative location and orientation of genes. b Diagram of eCPX3.0 gene from 5’ to 3’ indicating location of 15-mer peptide display library sequence, restriction sites flanking the library, and the location of the C-terminal P2X tag. c Schematic of mature eCPX3.0 display scaffold embedded in the cell membrane with N-terminal peptide library (preceded by CTSGQ) and C-terminal P2X tag displayed at the cell surface, and DsRed accumulating in the cytosol for cell visualization. The N-terminal peptide library contains random sequences, while the fixed C-terminal P2X tag allows expression monitoring through binding to the fluorescent protein YPet-Mona