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Fig. 1 | BMC Biotechnology

Fig. 1

From: Tuning the performance of CAR T cell immunotherapies

Fig. 1

Diagrams of different approaches to genome editing. a CRISPR/Cas9 uses guide RNA (gRNA) to hybridize to a specific site in the genome and cleave the genomic DNA. b The Sleeping Beauty transposon aligns terminal repeat DNA sequences with target DNA prior to DNA cleavage and break repair to generate a T/A dinucleotide repeat at the site of initial cleavage. c Zinc finger (ZF) nuclease is shown with 3 finger domains recognizing either half site. d TALEN is shown to consist of nuclease (N) and protein domains that each recognize a unique base pair. The images are original depictions, not intended as precise molecular models of the proteins and nucleic acids involved in the reactions

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