Table 1 Summary of secretion and surface display integrative vector components
Plasmid constructs | Size (bps) | Promoter | Signal peptide | Propeptide | LPXTG anchor domain | Function |
|---|---|---|---|---|---|---|
pS1 | 3111 | P170 | USP45 | LEISSTCDA | – | Secretion |
pS2 | 3072 | P170 | SPK1 | – | – | Secretion |
pS3 | 3144 | PnisA | USP45 | LEISSTCDA | – | Secretion |
pS4 | 3086 | PnisA | SPK1 | – | – | Secretion |
pSD1 | 4152 | P170 | USP45 | LEISSTCDA | PrtP344 | Surface display |
pSD2 | 4107 | P170 | SPK1 | – | PrtP344 | Surface display |
pSD3 | 4185 | PnisA | USP45 | LEISSTCDA | PrtP344 | Surface display |
pSD4 | 4146 | PnisA | SPK1 | – | PrtP344 | Surface display |