Fig. 7From: Rapid generation and selection of Cas9-engineering TRP53 R172P mice that do not have off-target effectsFunctional confirmation of the UV radiation hypersensitivity of TRP53 R172P. a The agarose gel image shows the PCR identification of mouse embryonic fibroblast (MEF) cells from 14 E13.5 embryos from the mating via TRP53 R172P heterozygous male and female mice. b Sanger DNA sequencing of PCR products identified the wild type (WT), TRP53 R172P heterozygous (HET) and homozygous (HOM) MEFs. c Western blotting shows the expression level of p53 and p21 in WT (#1), HET (#9) and HOM (#8) MEFs, suggesting the UV radiation hypersensitivity of TRP53 R172P HOMBack to article page