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Fig. 5 | BMC Biotechnology

Fig. 5

From: Rapid generation and selection of Cas9-engineering TRP53 R172P mice that do not have off-target effects

Fig. 5

Analysis of the off-target effects in the descendants of mice with or without off-target effects. a The #1 off-target locus (Trp53 pseudogene) in 8 descendants of mice with off-target effect (mouse# KI3) was PCR amplified with the specific primer pair and separated with electrophoresis on agarose gel. b Sanger Sequencing and BLAST showed the representative off-target effect detected in one offspring (mouse #6p202). c Sanger Sequencing and BLAST showed the representative on-target effect detected in one offspring (mouse #6p204). d The #1 off-target locus (Trp53 pseudogene) in 8 descendants of mice with on-target effect (mouse #6p204) was PCR amplified and assayed on agarose gel. e Sanger Sequencing and BLAST showed the representative on-target effect (mouse #6p301) detected in all 8 descendants of mice with on-target effect (mouse #6p204)

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BMC Biotechnology

ISSN: 1472-6750

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