Fig. 3

Validation of the TRP53 R172P KI in various tissues of mice and in the mice of Generation 1. a The electropherogram shows the PCR identification of various tissues of KI mice with the product of 607 bp. b Sanger DNA sequencing of PCR products confirmed the desired mutations in the different tissues including brain, heart, ovary, stomach, tail, spleen, tongue, liver, kidney, thymus, colon and lung. c The agarose gel image shows the PCR identification of Generation 1 offspring of Cas9 engineered mice with the product of 607 bp. d DNA sequencing of PCR products confirmed the expected mutations in the genome of G1 mice