Disruption of a putative mitochondrial oxaloacetate shuttle protein in Aspergillus carbonarius results in secretion of malic acid at the expense of citric acid production

Table 1 Transcription analysis of A. niger genes orthologous to the mitochondrial transporters identified in A. carbonarius

A. carbonarius ITEM 5010	Protein ID in A. carbonarius (JGI genome)	locus tag in A. niger	A. niger H915–1					A. niger AB 1.13
			6 h_FPKM	12 h_FPKM	24 h_FPKM	36 h_FPKM	48 h_FPKM	75 h_RPKM	75 h_RPKM
			6 h	12 h	24 h	36 h	48 h	75 h	92 h
			citric acid titer at point of sampling (g/l)
			≈ 3	≈ 18	≈ 50	≈ 86	≈ 118	2.5	7
ctp1 (ctpA)	139563	An11g11230	387.65	248.98	245.78	253.95	268.37	139,82	141,61
ctp1 (ctpB)	515063	An18g00070	3.70	0.83	3.24	0.54	1.18	0,54	0,56
yhmA	398055	An09g06670	605.39	406.06	315.49	532.38	395.27	386,26	329,29
yhmB	399724	An02g11090	36.37	55.64	9.04	41.48	76.31	0.07	0.06
mtpA	209833	An14g06860	70.50	35.61	44.76	38.02	38.64	58,62	59,27

A. niger H915–1- a citric acid producing strain [28]; A. niger AB1.13 – a commonly laboratory strain [20]. RPKM (Reads Per Kilobase of target per Million mapped reads) and FPKM (Fragments Per Kilobase of target per Million mapped reads) values were calculated according to the method presented by Mortazavi et al. [29], in order to normalize data for gene length

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