Target | Primers | Sequences | Amplicon (bp) | References |
---|---|---|---|---|
Actin = Plant reference gene | Actin | Forward 5′-CAAGCAGCATGAAGATCAAGGT-3’ | 103 | [24] |
Reverse 5′-CACATCTGTTGGAAAGTGCTGAG-3’ | ||||
Probe 5′-HEX-CCTCCAATCCAGACACTGTACTTYCTCTC-BHQ-3’ | ||||
CaMV Promoter 35S | P35S | Forward 5′-CGTCTACAAAGCAAGTGGATTG-3’ | 79 | [25] |
Reverse 5′-TCTTGCGAAGGATAGTGGGATT-3’ | ||||
Probe 5′-FAM-TCTCCACTGACGTAAGGGATGACGCA-QSY-3′ | ||||
CaMV gene P3 | P3 | Forward 5′-TGAAATCCTCAGTGACCAAAAATC-3′ | 152 | [23] |
Reverse 5′-TACAAGGACAATCATTGATGAGC-3′ | ||||
Probe 5′-ABY-AAGCCGTTGCAGCGAAAATCGTTAATGA-QSY-3’ | ||||
A.tumefaciens nopaline synthase terminator | TNOS | Forward 5′-GTCTTGCGATGATTATCATATAATTTCTG-3’ | 151 | [24] |
Reverse 5′-CGCTATATTTTGTTTTCTATCGCGT-3’ | ||||
Probe 5′-JUN-AGATGGGTTTTTATGATTAGAGTCCCGCAA-QSY-3′ |