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Table 1 Overview of the studied protective elements and their ability to sustain transgene expression in CHO-K1 cells

From: Sustained transgene expression from sleeping beauty DNA transposons containing a core fragment of the HNRPA2B1-CBX3 ubiquitous chromatin opening element (UCOE)

Group

Element size (kb)

No. of clones analyzed

Day 0 MFIa

Day 0 interclonal variationa

Day 0:Day 49 eGFP ratioa

Stable clones (%)b

CGIP

–

12

14,549 ± 0.3129

8.238 ± 1.772

0.56 ± 0.12

42

cHS4

2 × 1.2

14

24,838 ± 0.3454

2.559 ± 0.3559

0.79 ± 0.16

71

D4Z4

3.3

7

11,265 ± 0.2904

3.035 ± 0.7825

0.56 ± 0.10

43

5’1.5UCOE

1.5

14

17,014 ± 0.2988

6.358 ± 1.117

0.97 ± 0.01

100

3’1.5UCOE

1.5

16

17,085 ± 0.3009

10.97 ± 1.932

0.78 ± 0.08

63

5’UCOE-CORE

0.86

13

20,498 ± 0.5042

4.790 ± 1.178

0.46 ± 0.11

31

3’UCOE-CORE

0.86

13

20,256 ± 0.3585

4.454 ± 0.7882

0.87 ± 0.08

77

  1. amean ± SEM, b eGFP ratio ≥ 0.8