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Fig. 2 | BMC Biotechnology

Fig. 2

From: Sustained transgene expression from sleeping beauty DNA transposons containing a core fragment of the HNRPA2B1-CBX3 ubiquitous chromatin opening element (UCOE)

Fig. 2

Robust protection from transcriptional silencing mediated by cHS4 and 1.5UCOE elements in Sleeping Beauty transposon vectors. Schematic illustration of SB transposon vector constructs and analysis of their respective analysis of eGFP expression in single CHO-K1 clones over a 7 week period of continuous culture in the absence of puromycin selective pressure with either cHS4 (a), D4Z4 (b) or the 1.5UCOE in a 5′- (c) or 3′- (d) orientation. Each line in the graphs represents the expression profile of a single clone over the course of the 7 weeks of continuous culture

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