Fig. 7

CE analysis of complex formation between GFLV CP VLPs (A) or GFLV L2 VLPs (B) and GFLV antibody. a VLPs analysis without antibody, b VLPs incubated with 200 fold molar excess of GFLV antibody during one hour at room temperature before injection. CE analysis of complex formation between GFLV CP VLPs (C) or GFLV L2 VLPs (D) and HPV L2 antibody. a VLPs analysis without antibody, b VLPs incubated with 39 fold molar excess of HPV L2 antibody during one hour at room temperature before injection. Peak 1, IS; peak 2, VLP; peak 3, GFLV L2 VLPs / HPV L2 antibody complex; BGE, 0.01 M Tris HCl, 0.01 M HEPES-Na, 0.1 M NaCl and 0.1% PEG 6000, pH 7.4 buffer containing 0.2% Tween 20 and 1.5 mM SDS; 48.5 cm (8.5 cm to the detector) × 50 μm PEO-coated capillary; voltage + 10 kV; pressure injection for 15 s at − 50 mbar; T = 15 °C; Detection at 280 nm