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Fig. 3 | BMC Biotechnology

Fig. 3

From: Production and characterization of virus-like particles of grapevine fanleaf virus presenting L2 epitope of human papillomavirus minor capsid protein

Fig. 3

Western blot analysis of the GFLV CP and GFLV L2 VLPs expressed in E. coli (a and b) and in P. pastoris (c and d). a and c: blots were probed with anti-GFLV CP polyclonal antibody. Lane M: standard protein molecular weight markers, lane 1: GFLV CP, lane 2: GFLV L2, lane 3: negative control (E. coli with pET26 empty vector or P. pastoris with pPICZα empty vector). b and d: blots were probed with mouse anti-HPV L2 antibody. Lane M: standard protein molecular weight markers, lane 1: GFLV L2, lane 2: GFLV CP

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