Fig. 3

Representative examples of large insertions (≥20 bp) for gRNA2 (replicate 3). Schematics of pUbi-Cas9-rbcS (top) and pCR8-U6-gRNA2 (bottom) are shown, annotated with links (dotted lines) indicating from where the inserted sequences originate. Bold black text, PAM; blue text, complimentary to gRNA2 guide sequence; red text, inserted sequence. The downward-pointing indicates the position of the canonical cut site. The type/size of the mutation is given in brackets, together with the allele frequency as a percent of edited amplicon reads