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Fig. 2 | BMC Biotechnology

Fig. 2

From: gRNA validation for wheat genome editing with the CRISPR-Cas9 system

Fig. 2

Mutation detection and summary of editing efficiencies for seven gRNAs targeting EPSPS on chromosomes 7AS, 4AL and 7DS. a TIDE detection of mixed peaks in the reverse Sanger sequence read for gRNA5 on chromosome 7AS (replicate 1). b Summary of TIDE results. N.D., not detected. n.s., not statistically significant. Error bars represent the standard error of the mean (n = 3). c Alignment of representative mutant amplicon reads for gRNA2 on chromosomes 7AS, 4AL and 7DS (replicate 1). Bold black text, PAM; blue text, complementary to gRNA2 guide sequence; red text, inserted nucleotide. Downward-pointing arrow heads indicate the position of the canonical cut site. The number of reads and percent of total reads is shown in brackets. d Summary of CRISPResso results. Error bars represent the standard error of the mean (n = 3). * statistically significant (p < 0.05) based on a two-sample t-test assuming unequal variances. The keys in b also apply to d. In the key for guide sequence mismatches, p20 means position 20 in the guide sequence, etc.

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