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Fig. 1 | BMC Biotechnology

Fig. 1

From: gRNA validation for wheat genome editing with the CRISPR-Cas9 system

Fig. 1

Target sites for seven gRNAs targeting EPSPS. The gene structure of EPSPS is shown, annotated with the universal mutation hotspot for glyphosate resistance (yellow asterisk in exon 2). The detail underneath shows partial, abbreviated sequences for the three homoeoalleles of EPSPS on chromosomes 7AS, 4AL and 7DS. Target sites are indicated by coloured bars. PAM sites (5′-NGG-3′) are indicated by black bars at the ends of the coloured bars. Downward-pointing arrow heads indicate the position of the canonical cut site and predicted specificity based on the number and distribution of homoeologous SNPs at the corresponding target site/PAM

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