Fig. 7

Transcriptional GUS activity in transgenic A. thaliana under 12 h of ABA (a), PEG (b) or drought (c) treatments. Levels of uidA mRNA of non-treated (control) and treated transgenic plants were measured using real-time PCR. The expression levels were normalized using ACT2 and GAPDH as endogenous controls. The relative gene expression was calculated using the 2^-ΔΔCt method. The bars represent standard error and the asterisks (*) indicate statistical significance determined by Student’s t-test (p ≤ 0.05). The data shown are representative of three independent lines (n = 3)