Table 3 Comparison of rHTTP method with few commercially available kits
Kits/methods | Time required per sample | Instrumentation | Cost |
|---|---|---|---|
DNeasy Plant Mini Kit (Qiagen) | ~ 60 min | Grinding assembly/ Mortar-pestle, Centrifuge, vortex (6 centrifugation steps) | INR 282/− per prep |
Synergy™ 2.0 (OPS Diagnostics) | 43 min | Centrifuge, vortex (5 centrifugation steps) | INR 153/− per prep |
Plant DNA isolation reagent (Takara) | 30 min. | Centrifuge, vortex (3 centrifugation steps) | INR 138/− per prep |
Phire Plant Direct PCR Kit (Thermo Fisher Scientific) | – | Thermal cycler (sample lysis and PCR done in a single tube). Crushing and spinning required in Dilution protocol | INR 51/− per prep |
QuickExtract™ Plant DNA extraction solution (Lucigen) | 8 min | Heating device with temperature controller (it requires incubation at two different temperatures) | INR 40/− per prep |
rHTTP method | 10 min | Thermal cycler/ heating device/ any means to boil water | INR 4.5/− per prep |