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Table 2 Comparison of rHTTP method with other reported methods of quick plant DNA extraction

From: Rapid high throughput template preparation (rHTTP) method: a novel cost effective method of direct PCR for a wide range of plants

  Rapid One Step Extraction (ROSE) method (Steiner et al., 1995) Sucrose Prep Method (Berendzen et al. 2005) NaOH Method (Wang et al., 1993) NaOH Method (Satya et al. 2013) NaOH Method (Wang et al., 2015) rHTTP method (Present study)
Type and form of plant material Lyophilized plant tissue Fresh tissue Young tissue Fresh tissue Fresh tissue Fresh/old tissue
Liquid nitrogen No Yes/Ice No No No No
Extraction/lysis buffer Tris-HCl, EDTA, Sodium Sarkosyl sulfate, PVPP Tris-HCl, NaCl, Sucrose NaOH, Tris NaOH, Tris-EDTA NaOH SDS
Instrumentation Lyophilizer, Shaker, hybridization Oven/ Water bath Water Bath/Thermal cycler, Centifuge, grinding assembly/Mortar pestle Grinding assembly/ Mortar-pestle Grinding assembly/ Mortar-pestle, Centrifuge Grinding assembly/ Mortar-pestle/ centrifuge Thermal cycler/hot plate/any means to boil water
Duration to make templates for PCR (as per original publication) ~ 45 min after lyophilization ~ 15 min Should not be more than 10 min 10–12 min 5 min 10 min
Actual time required to process five samples at a time ND 59 min
(11.8 min per sample)
*For larger number of samples time will increase proportionately due to grinding
31 min
(6.2 min per sample)
*For larger number of samples time will increase proportionately due to grinding
65 min
(13 min per sample)
*For larger number of samples time will increase proportionately due to grinding
29 min
(5.8 min per sample)
*For larger number of samples time will increase proportionately due to grinding
12.5 min
(2.5 min per sample)
*For larger number of samples, the time will remain almost same as there is no grinding or maceration. The time will be 10 min plus time required for pipetting 100 μl lysis buffer