Table 1 DNA quality and yield in lysates prepared with different concentrations of SDS, different amount of tissue and different time duration
Parameters | DNA Purity (A260/A280) | DNA Concentration (ng/μl) a | |
|---|---|---|---|
SDS conc. (%) | 0.5 | 1.03 | 451.67 ± 8.82 |
1.0 | 1.01 | 768.33 ± 6.01 | |
1.5 | 1.03 | 376.67 ± 4.41 | |
2.0 | 1.04 | 252.17 ± 1.48 | |
2.5 | 1.03 | 438.33 ± 14.24 | |
3.0 | 1.04 | 261.17 ± 21.17 | |
3.5 | 1.05 | 218.33 ± 3.33 | |
4.0 | 1.06 | 260.17 ± 3.06 | |
4.5 | 1.05 | 326.67 ± 1.67 | |
5.0 | 1.04 | 446.67 ± 14.81 | |
Amount of tissue | One disc (~ 1.6 mg) | 1.06 | 420.00 ± 10.08 |
Two disc(~ 3.2 mg) | 1.04 | 760.00 ± 6.75 | |
Time durations | 10 min | 1.08 | 876.67 ± 37.12 |
20 min | 1.05 | 808.33 ± 6.01 |