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Table 1 DNA quality and yield in lysates prepared with different concentrations of SDS, different amount of tissue and different time duration

From: Rapid high throughput template preparation (rHTTP) method: a novel cost effective method of direct PCR for a wide range of plants

Parameters   DNA Purity (A260/A280) DNA Concentration (ng/μl) a
SDS conc. (%) 0.5 1.03 451.67 ± 8.82
1.0 1.01 768.33 ± 6.01
1.5 1.03 376.67 ± 4.41
2.0 1.04 252.17 ± 1.48
2.5 1.03 438.33 ± 14.24
3.0 1.04 261.17 ± 21.17
3.5 1.05 218.33 ± 3.33
4.0 1.06 260.17 ± 3.06
4.5 1.05 326.67 ± 1.67
5.0 1.04 446.67 ± 14.81
Amount of tissue One disc (~ 1.6 mg) 1.06 420.00 ± 10.08
Two disc(~ 3.2 mg) 1.04 760.00 ± 6.75
Time durations 10 min 1.08 876.67 ± 37.12
20 min 1.05 808.33 ± 6.01
  1. aDNA concentration is represented as Mean ± SE