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Fig. 4 | BMC Biotechnology

Fig. 4

From: Development of a novel human phage display-derived anti-LAG3 scFv antibody targeting CD8+ T lymphocyte exhaustion

Fig. 4

Construction and characterization of the divalent scFvF7-Fc Ab. a Scheme of the vector expressing the scFvF7-Fc open reading frame. Indicated are the locations of the hEF1α/HTLVI promoter, IL-2 signal sequences (IL-2 SS) acting as signal peptide, multiple cloninig site (MCS), and hinge. Domain map of the resulting protein product is also illustrated and, on the right, the overall structure of the divalent Ab. b Anti 6 × histidine western blot analysis carried on supernatants of CHO cells transfected with either the scFvF7-Fc expressing vector (lane 1), or transfected with the empty vector (lane 2). Molecular weight markers (Mk) are reported on the left. The results are representative of four independent assays. c ELISA performed with supernatants of CHO cells transfected with the vector expressing scFvF7-Fc. Either recombinant LAG3 or glucose oxidase (GO) were coated in the microwells (0.5 μg). The mean values ±SEM are shown as calculated from results of three independent assays

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