Fig. 1

Isolation and screening of anti-LAG3 scFvs by phage display technology. a Scheme of the LAG3 affinity-based phage selection starting from the IORISS1 library. b Anti-LAG3 ELISA carried out on supernatants of 90 bacterial colonies isolated after five rounds of panning cycles. The plate was coated with 0.5 μg of antigen per microwell. The numbers identify each positive clone. O.D. nm 450 ref. 620: optical density