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Table 1 Agreement analysis between direct PCR sequencing and stuntmer PCR

From: A new primer construction technique that effectively increases amplification of rare mutant templates in samples

Exon 21 L858RTraditional PCRTotal
PositiveNot found
FFPE
 Stuntmer PCR
  Positive29156347
  Not found0935935
 Total2919911282
Pleural effusion
 Stuntmer PCR
  Positive593392
  Not found0226226
 Total59259318
Total35012501600
Positive percent agreement (95% CI)100% (98.9, 100%)
Negative percent agreement (95% CI)92.9% (91.3, 94.2%)
Overall percent agreement (95% CI)94.4% (93.2, 95.5%)
Exon 19 deletionTraditional PCRTotal
DeletionInsertionNot found
FFPE
 Stuntmer PCR
  Deletion2720140412
  Not found00870870
 Total272010101282
Pleural effusion
 Stuntmer PCR
  Deletion56055111
  Insertion0101
  Not found00206206
 Total561261318
Total328112711600
Positive percent agreement (95% CI)100% (98.8, 100%)
Negative percent agreement (95% CI)84.7% (82.6, 86.5%)
Overall percent agreement (95% CI)87.8% (86.1, 89.3%)
Exon 20 T790 MTraditional PCRTotal
PositiveNot found
FFPE
 Stuntmer PCR
  Positive163248
  Not found012341234
 Total1612661282
Pleural effusion
 Stuntmer PCR
  Positive2810
  Not found0308308
 Total2316318
Total1815821600
Positive percent agreement (95% CI)100% (82.4, 100%)
Negative percent agreement (95% CI)97.5% (96.6, 98.1%)
Overall percent agreement (95% CI)97.5% (96.6, 98.2%)