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Fig. 4 | BMC Biotechnology

Fig. 4

From: A new primer construction technique that effectively increases amplification of rare mutant templates in samples

Fig. 4

Different stuntmer detection sensitivity levels. The mutant detection sensitivity of the T790 stuntmer with the cfDNA Reference Standard Set was tested. Nested PCRs using the T790 stuntmer were able to effectively increase the mutant detection sensitivity of the stuntmer without affecting specificity. The primer successfully reversed the ratios of the mutant and wild-type sequences in the PCR products, allowing the detection of mutant templates that made up only 0.1% of the sample

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