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Fig. 1 | BMC Biotechnology

Fig. 1

From: A new primer construction technique that effectively increases amplification of rare mutant templates in samples

Fig. 1

Stuntmer structure and binding conformations to wild-type and mutant templates. a The stuntmer primer consists of a recognition (R) region, a linker region, and an extension (E) region. The wild-type template has two sites that can bind to the stuntmer (b, c). When the R region is bound to the template, the E region remains unbound, and the wild-type template remains unamplified. The R region binding to mutant templates is unstable because of the mismatch between the two sequences (d). This allows the E region to bind to mutant templates (e), and allows amplification of mutant templates

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