Fig. 3

a Reactivity of the anti-VP35 scFv clones in ELISA. Either the recombinant VP35 (blue bars) or the recombinant glucose oxidase (GO, red bars) were used as antigens. The anti-GO scFv was used as control (ctrl) for detection in parallel with the anti-VP35 scFvs. The mean values and the SD of a representative experiment out of at least three, performed in triplicate, are reported. b Reactivity of the anti-VP35 scFv clones in immunoblot assay. Recombinant VP35 protein and GO antigen as a control were analyzed by SDS-PAGE, blotted onto nitrocellulose membrane and cut into strips. Each strip was incubated with the supernatant deriving from a bacterial clone producing one of the anti-VP35 scFvs as indicated, and the antigen-antibody reaction revealed by ECL. The GO antigen detection by ctrl scFv and the recombinant VP35 detection by anti-His mAb, were used as positive controls. The molecular weights (MW) of the bands corresponding to GO and VP35 protein are indicated by arrows