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Fig. 1 | BMC Biotechnology

Fig. 1

From: Intracellular human antibody fragments recognizing the VP35 protein of Zaire Ebola filovirus inhibit the protein activity

Fig. 1

Reactivity of the scFvs against the recombinant EBOV VP35 in ELISA. The IPTG-induced bacterial supernatants of individual colonies from the third round of selection were tested in 96-well microtiter plates coated with the recombinant VP35 protein as an antigen. The cut-off value separating positive from negative samples was calculated as 3 standard deviation values (SD) above the mean, of the values obtained using the irrelevant anti-GO scFv (OD450 = 0.079). The five different positive clones isolated, identified by sequence analysis, are indicated

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BMC Biotechnology

ISSN: 1472-6750

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