Table 2 Kinetic parameters of wild-type, mutated and combined mutations Ph-HepI
From: Structure-based engineering of heparinase I with improved specific activity for degrading heparin
Enzyme | Km (mM) | kcat (s− 1) | kcat/Km (s− 1 M− 1 × 10− 3) |
|---|---|---|---|
Wild type | 0.78 ± 0.05 | 19.31 ± 0.17 | 24.76 |
S169D | 0.25 ± 0.07 | 23.19 ± 0.09 | 92.76 |
A259D | 0.33 ± 0.09 | 21.56 ± 0.15 | 65.33 |
S169D/A259D | 0.21 ± 0.06 | 26.32 ± 0.03 | 125.33 |