Fig. 5
From: Structure-based engineering of heparinase I with improved specific activity for degrading heparin
The purified protein was analyzed by 12% SDS-PAGE and stained with Coomassie Brilliant Blue R-250. Lane M—protein marker; lanes 1, 3, 5, 7—wild-type Ph-HepI and S169D, A259D and S169D/A259D mutants are produced by inducing the recombinant bacteria; lanes 2, 4, 6, 8—purified wild-type Ph-HepI and S169D, A259D and S169D/A259D mutants. The band of Ph-HepI protein (53 kDa) is indicated by an arrow