Characterization of tethered equine chorionic gonadotropin and its deglycosylated mutants by ovulation stimulation in mice

Table 1 Superovulation of mouse oocytes between natural-eCG and tethered rec-eCGβ/α deglycosylated mutants

	No. Oocytes
	Functional	Nonfunctional	Nonfunctional relative to total oocytes (%)
PMSG (n = 9)	22.6 ± 2.9A	4.78 ± 0.5a	21.2
rec-eCGβ/α (n = 6)	13.5 ± 1.3B	0.33 ± 0.2b	2.4
rec-eCGβ/αΔ56 (n = 6)	14.8 ± 1.2B	0b	0
rec-eCGβ-D/α (n = 6)	14 .0 ± 1.8B	0.33 ± 0.3b	2.4
rec-eCGβ-D/αΔ56 (n = 6)	15.0 ± 0.6B	0b	0

The mice were superovulated by injection of 10 IU of PMSG or rec-eCGs and then 10 IU hCG after 48 h, and ovulated oocytes were collected into an oviduct ampulla after 13 h as described in Methods. 1 IU was assumed to be 100 ng according to the conversion factor of the suggested assay protocol corresponding PMSG by standard curve. The ovulated oocytes were determined under microscope as shown in Additional file 1: Figure S1. The percentage of nonfunctional relative to total oocytes was around 20% with natural eCG and only around 2% with rec-eCGs used
Values are expressed as mean ± SEM for at least three independent experiments
A-B, a-bValues with different superscripts are significantly different (p < 0.05)

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