Fig. 3

Western blot analysis of tethered rec-eCG mutants. a Rec-eCG mutants were expressed in CHO-K1 cells. The proteins in conditioned media were collected, separated by SDS-PAGE, and transferred to a blotting membrane. The proteins were detected with antibodies against the myc-tag. b Proteins for western blotting were also treated with N-GlycosidaseF. Lane 1: β/α, Lane 2: β/αΔ56, Lane 3: β-D/α, Lane 4: β-D/αΔ56,-: not treated, +: treated with N-Glycosidase-F