Table 4 PCR primers for 35S promoter amplification. The M35F primer is derived from Wu et al. 2014 [20] and the M3R is from Fernandez et al. 2005 [21]. Both primers were synthesised by Sigma with HPLC purification
From: Lack of specificity associated with using molecular beacons in loop mediated amplification assays
Target, Type, Notation | Primer Sequence (5’ to 3’) |
|---|---|
35Sp, Forward, M25F | CATCATTGCGATAAAGGAAAGGC |
35Sp, Reverse, M3R | TCTTGCGAAGGATAGTGGGATT |