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Fig. 9 | BMC Biotechnology

Fig. 9

From: Lack of specificity associated with using molecular beacons in loop mediated amplification assays

Fig. 9

LAMP quantification with molecular beacon and SYTO9. The 35S promoter STEM 5nt molecular beacon was used with a serial dilution of an optimised artificial template in (1) and the same template dilutions were used with SYTO9 detection in (2). Normalised fluorescence for the dilutions series and NTC (A), Melt curve analysis for the dilution series and NTC (B) and average Ct against template dilution for the dilution series (C). The threshold was set at 0.05 for the LAMP MB and 0.4 for the SYTO9 detection. The peaks in 1B for the LAMP molecular beacon melt curve were inverted

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