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Fig. 6 | BMC Biotechnology

Fig. 6

From: Lack of specificity associated with using molecular beacons in loop mediated amplification assays

Fig. 6

Fluorescent signal from MB-LAMP with looped or linear DNA.35Sp oligonucleotide designed for ‘dumbbell’ looped structure undiluted (1A) and ten-fold dilution (1B) in blue, NTC in orange. Fluorescence from LBP molecular beacon in real-time at 60 degrees C for 5 min and melt curve analysis. Average fluorescence and melt temperature derived from replicates. 35Sp oligonucleotide of the same length without loops undiluted (2A) and ten-fold diluted (2B) in blue with NTC in orange. Fluorescence acquisition from LBP molecular beacon at 60 degrees C for 5 min and melt curve analysis

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