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Fig. 5 | BMC Biotechnology

Fig. 5

From: Lack of specificity associated with using molecular beacons in loop mediated amplification assays

Fig. 5

Lack of enhanced specificity with LBP LAMP molecular beacon. LAMP amplification of 35Sp template in blue and non-template control in orange with detection of amplification with a FAM fluorophore labelled molecular beacon designed for the ompW gene of Vibrio chlorerae [10]. Fluorescent output against cycles of 60 seconds at 60 degrees C and melt curve analysis post amplification

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