Fig. 3
From: Lack of specificity associated with using molecular beacons in loop mediated amplification assays
Amplification curves of 35Sp 5nt STEM molecular beacon at different temperatures. LAMP assay temperature incrementally from 55 degrees C to 65 degrees C for positive template and NTC, (a) shows the increase in fluorescence of the FAM fluorophore attached to the 35Sp 5nt STEM molecular beacon at various temperatures and (b) shows the corresponding NTCs for each assay. The positive and negative samples at various temperatures for the 35Sp 4nt STEM MB, 35Sp 6nt STEM MB and for 35Sp 7nt STEM MB are shown in Additional file 1: Figure S1