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Fig. 2 | BMC Biotechnology

Fig. 2

From: Lack of specificity associated with using molecular beacons in loop mediated amplification assays

Fig. 2

LAMP amplification with 35Sp LoopF molecular beacon. LAMP primers designed to amplify part of the 35S promoter sequence detected by fluorescence from a LAMP molecular beacon targeting the LoopF sequence. 1A shows the fluorescence for dilutions of the 35Sp LAMP MB; 125 nanomolar dark blue, 12.5 nanomolar light blue, 1.25 nanomolar light green, 125 picomolar pink and NTC in orange. 2A denotes the fluorescent output against cycles of 1 min at 63 degrees C for 35Sp LAMP amplification excluding the LoopF oligonucleotide (positive samples with template in blue and NTC in orange). 3A charts the fluorescent output against cycles of 1 min at 63 degrees C for 35Sp LAMP amplification with the inclusion of the LoopF oligonucleotide. LAMP molecular beacon used at a concentration of 0.32 micromolar for these two assays. Melt curve analysis shown in 1B to 3B with peaks inverted

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