Fig. 3

Multiplexed LASSO capture NGS data analysis a Distribution of reads aligned to E. coli genome and LASSO probe backbone reference sequences. Unaligned reads were also included to reflect total raw sequencing data. b Target enrichment values were derived from the ratio of median normalized read depths of targets over median normalized read depths of non-targets. Statistical significance of target enrichment over non-targets was determined using Student’s t-test with a 0.99 confidence interval (p-value < 0.01. c The lengths of LASSO capture targets longer than 1.0 kb with full sequencing coverage were normalized to 1 (Base-pair position normalization). The sequenced depth for each base in captures was then plotted for each normalized position along gene to visualize for target enrichment levels and capture efficiency