Fig. 3
From: Crosslinked flagella as a stabilized vaccine adjuvant scaffold
Crosslinking and stability of cysteine-substituted flagella. a Extracted filaments subjected to SDS-PAGE with and without β-mercaptoethanol (β-ME) reduction signifying the presence of disulfide bonds in all three cysteine-substituted flagella. Image representative of three independent experiments. b Thermal and acidic filament stability assessed by Native-PAGE following treatment with 50 mM HCl or heating at 60 °C for 10 min compared to untreated (UT). Image representative of two independent experiments. c Densitometry quantitation of replicate Native-PAGE gels as shown in b. Data included from two independent experiments with standard deviation shown