Fig. 2
From: Crosslinked flagella as a stabilized vaccine adjuvant scaffold
Assessing the structure and function of cysteine-substituted flagella. a Fluorescent staining of S. Typhimurium shows production and structural appearance of the respective flagella. Images included from three independent experiments. b Measurements of helical curvature (μM− 1) and twist (μM− 1) from each modified flagella compared to theoretical helical structures (red symbols). Multiple measurements were taken from both attached and detached filaments. Data (n ≥ 6) from two independent experiments. c Soft-agar motility assay depicting the effect of cysteine substitutions on the swimming motility of S. Typhimurium. Image representative of three independent experiments