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Fig. 1 | BMC Biotechnology

Fig. 1

From: Preparation of a novel monoclonal antibody against caprine interleukin-17A and its applications in immunofluorescence and immunohistochemistry assays

Fig. 1

Enlarged cultivation and purification of the recombinant cIL-17A induced at 16 °C for 42 h. M, Premixed protein marker (low); Lane 1, the uninduced cIL-17-PET 32a-TransB (DE3); Lane 2, induced PET 32a-TransB (DE3) with IPTG at a concentration of 0.5 mmol/L; Lane 3, unpurified recombinant cIL-17A; Lane 5–7, eluents after the unpurified supernatant flowed through the column; Lane 8–10, eluents of unrelated proteins after the column was washed with imidazole at a concentrations of 80 mmol/L; Lane 11 and 12, purified recombinant cIL-17A. The position of the recombinant fusion protein is indicated by an arrow

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