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Table 2 Strains and plasmids used in this study

From: Improved the expression level of active transglutaminase by directional increasing copy of mtg gene in Pichia pastoris

Strains Genotype References
E. coli TOP10 F-mcrAΔ (mrr-hsdRMS-mcrBC) φ80 lacZΔM15 ΔlacX74 recA1 araΔ139 Δ (ara-leu)7697 galUgalKrpsL (StrR) endA1 nupG. used for vector sub-cloning Invitrogen
Pichia pastoris GS115 his4,host strain Invitrogen
GS115 (pro) The pro-peptide of MTG was integrated into his4 site of Pichia pastoris GS115 This study
GS115(pro/rDNA-mtg) The mature sequence of MTGwas integrated into non-coding rDNA site ofGS115 (pro) This study
Plasmids Description Reference
pPICZα-B Containing AOX1 promoter for tightly regulated, methanol-induced expression of the gene, ZeocinR Invitrogen
pGAP9 pPIC9 derivative, GAP promoter instead of AOX1 promoter Lab stock
pGAP9-pro pGAP9 derivative carrying an internal 135 bp fragment of pro gene This study
pPICZα-rDNA-mtg pPICZα-mtg derivative carrying an internal 1153 bp fragment of rDNA gene, ZeocinR This study