Table 2 Strains and plasmids used in this study
Strains | Genotype | References |
|---|---|---|
E. coli TOP10 | F-mcrAΔ (mrr-hsdRMS-mcrBC) φ80 lacZΔM15 ΔlacX74 recA1 araΔ139 Δ (ara-leu)7697 galUgalKrpsL (StrR) endA1 nupG. used for vector sub-cloning | Invitrogen |
Pichia pastoris GS115 | his4,host strain | Invitrogen |
GS115 (pro) | The pro-peptide of MTG was integrated into his4 site of Pichia pastoris GS115 | This study |
GS115(pro/rDNA-mtg) | The mature sequence of MTGwas integrated into non-coding rDNA site ofGS115 (pro) | This study |
Plasmids | Description | Reference |
pPICZα-B | Containing AOX1 promoter for tightly regulated, methanol-induced expression of the gene, ZeocinR | Invitrogen |
pGAP9 | pPIC9 derivative, GAP promoter instead of AOX1 promoter | Lab stock |
pGAP9-pro | pGAP9 derivative carrying an internal 135 bp fragment of pro gene | This study |
pPICZα-rDNA-mtg | pPICZα-mtg derivative carrying an internal 1153 bp fragment of rDNA gene, ZeocinR | This study |