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E. coli TOP10
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F-mcrAΔ (mrr-hsdRMS-mcrBC) φ80 lacZΔM15 ΔlacX74 recA1 araΔ139 Δ (ara-leu)7697 galUgalKrpsL (StrR) endA1 nupG. used for vector sub-cloning
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Invitrogen
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Pichia pastoris GS115
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his4,host strain
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Invitrogen
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GS115 (pro)
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The pro-peptide of MTG was integrated into his4 site of Pichia pastoris GS115
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This study
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GS115(pro/rDNA-mtg)
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The mature sequence of MTGwas integrated into non-coding rDNA site ofGS115 (pro)
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This study
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Plasmids
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Description
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Reference
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pPICZα-B
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Containing AOX1 promoter for tightly regulated, methanol-induced expression of the gene, ZeocinR
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Invitrogen
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pGAP9
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pPIC9 derivative, GAP promoter instead of AOX1 promoter
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Lab stock
|
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pGAP9-pro
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pGAP9 derivative carrying an internal 135 bp fragment of pro gene
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This study
|
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pPICZα-rDNA-mtg
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pPICZα-mtg derivative carrying an internal 1153 bp fragment of rDNA gene, ZeocinR
|
This study
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