Table 1 Copy numbers of gap and mtg gene detected by real-time fluorescent quantitative PCR
Strain | Value Ct | Gene copy(10n) | Number of copies of mtg gene in P. Pichia genome (mtg copy number/gap copy number) | ||
|---|---|---|---|---|---|
mtg gene | gap gene | mtg gene | gap gene | ||
1 | 14.14 ± 0.27 | 16.49 ± 0.17 | 5.58 ± 1.02 | 1.66 ± 0.19 | 3.36 ± 0.24 |
2 | 14.93 ± 0.28 | 16.67 ± 0.22 | 3.29 ± 0.50 | 1.48 ± 0.18 | 2.21 ± 0.11 |
3 | 14.97 ± 0.30 | 18.23 ± 0.23 | 3.20 ± 0.63 | 5.60 ± 0.71 | 5.72 ± 0.35 |
4 | 15.30 ± 0.41 | 19.05 ± 0.17 | 2.56 ± 0.55 | 3.36 ± 0.31 | 7.62 ± 1.11 |