|
Strain
|
Value Ct
|
Gene copy(10n)
|
Number of copies of mtg gene in P. Pichia genome (mtg copy number/gap copy number)
|
|---|
|
mtg gene
|
gap gene
|
mtg gene
|
gap gene
|
|---|
|
1
|
14.14 ± 0.27
|
16.49 ± 0.17
|
5.58 ± 1.02
|
1.66 ± 0.19
|
3.36 ± 0.24
|
|
2
|
14.93 ± 0.28
|
16.67 ± 0.22
|
3.29 ± 0.50
|
1.48 ± 0.18
|
2.21 ± 0.11
|
|
3
|
14.97 ± 0.30
|
18.23 ± 0.23
|
3.20 ± 0.63
|
5.60 ± 0.71
|
5.72 ± 0.35
|
|
4
|
15.30 ± 0.41
|
19.05 ± 0.17
|
2.56 ± 0.55
|
3.36 ± 0.31
|
7.62 ± 1.11
|
- Datas are presented as mean ± SD of triplicate observations. Strain 1: the clone with the highest enzyme activity. Strain 2,3,4:another three positive clones
