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Table 1 Copy numbers of gap and mtg gene detected by real-time fluorescent quantitative PCR

From: Improved the expression level of active transglutaminase by directional increasing copy of mtg gene in Pichia pastoris

Strain Value Ct Gene copy(10n) Number of copies of mtg gene in P. Pichia genome (mtg copy number/gap copy number)
mtg gene gap gene mtg gene gap gene
1 14.14 ± 0.27 16.49 ± 0.17 5.58 ± 1.02 1.66 ± 0.19 3.36 ± 0.24
2 14.93 ± 0.28 16.67 ± 0.22 3.29 ± 0.50 1.48 ± 0.18 2.21 ± 0.11
3 14.97 ± 0.30 18.23 ± 0.23 3.20 ± 0.63 5.60 ± 0.71 5.72 ± 0.35
4 15.30 ± 0.41 19.05 ± 0.17 2.56 ± 0.55 3.36 ± 0.31 7.62 ± 1.11
  1. Datas are presented as mean ± SD of triplicate observations. Strain 1: the clone with the highest enzyme activity. Strain 2,3,4:another three positive clones