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Fig. 5 | BMC Biotechnology

Fig. 5

From: Improved the expression level of active transglutaminase by directional increasing copy of mtg gene in Pichia pastoris

Fig. 5

Detection of coexpression pro-peptide and MTG in GS115 (mtg-3c). a and b The pro-peptide were detected by SDS-PAGE and Western blot under non-reducing conditions. The proteins (pro-peptide) of 20 μl culture supernatant were separated by Tricine-SDS-PAGE and subjected to Western analysis using anti-His-HRP. c and d The protein (MTG) of 20 μl culture supernatant were separated by SDS- PAGE and subjected to Western analysis (anti-MTG).positive control: MTG from E.coli

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BMC Biotechnology

ISSN: 1472-6750

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